cd8a 168er  (fluidigm)

Journal: Cell Reports Medicine

Article Title: LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis

doi: 10.1016/j.xcrm.2025.102000

Figure Lengend Snippet: TGF-β blockade potentiates the efficacy of anti-PD-1 therapy in LTA4H knockout tumor-bearing mice (A) Tumor progression was monitored at 5, 9, and 13 days post inoculation using live bioluminescent imaging in mice injected with scramble or LTA4H KO Hepa1-6 cells and treated with IgG or blocking antibody as indicated. (B and C) Quantification of the liver tumor burden from live bioluminescent imaging studies. (D) Images of orthotopic HCC tumors from the indicated treatment groups. (E) H&E staining of Hepa1-6 orthotopic HCC tumors from treatment groups. Scale bar: 4 mm. (F) Comparison of tumor burden (tumor area/whole liver area) in orthotopic HCC tumors. (G) mIHC staining of CD206 + macrophages, PD-1 + CD8 + T cells, and GZMB + CD8 + T cells in Hepa1-6 scramble and LTA4H KO orthotopic HCC tumors with the indicated treatments. Scale bar: 20 μm. (H–K) Comparison of CD206 + macrophages, CD8 + T cells, PD-1 + CD8 + T cells, and GZMB + CD8 + T cells among Hepa1-6 scramble and LTA4H KO orthotopic HCC tumors with the indicated treatments. (L) mIHC staining of LTA4H, CD68, and CD206 in human HCC samples with varying responses to ICIs. Scale bar: 100 μm. (M and N) Comparison of LTA4H expression in HCC tumor cells and the percentage of CD206 + macrophages between responders ( n = 6) and non-responders ( n = 7). (B, C, F, and H–K) p values were calculated by one-way ANOVA with Tukey’s multiple comparison analysis ( n = 5), data represent mean ± SD. (M and N) p value was calculated by Wilcoxon rank-sum test, data represent mean ± SD. All the replicates represent biological replicates. See also Table S6 .

Article Snippet: CD8a; Mouse; 168Er , Fluidigm , 3168003B; RRID: AB_2811241.

Techniques: Knock-Out, Imaging, Injection, Blocking Assay, Staining, Comparison, Expressing

Journal: Cell Reports Medicine

Article Title: LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis

doi: 10.1016/j.xcrm.2025.102000

Figure Lengend Snippet:

Article Snippet: CD8a; Mouse; 168Er , Fluidigm , 3168003B; RRID: AB_2811241.

Techniques: Phospho-proteomics, Microarray, Recombinant, Lysis, Protease Inhibitor, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Reverse Transcription, Activity Assay, Immunoprecipitation, Extraction, Purification, In Vitro, Transfection, Sequencing, Mass Cytometry, ChIP-sequencing, Transgenic Assay, Software